Hygienic parameters

BT/TF 151

WI

Topic

Title

Final draft standard

Updated report

Validation data

Validation

Validation Study report E. coli and Salmonella spp. Inter-laboratory study

CSS99050-1    

EColi

Soils, sludges and treated bio-wastes — Detection and enumeration of Escherichia coli  – Part 1: Membrane filtration method for
quantification

CSS99050-2  

EColi

Soils, sludges and treated bio-wastes — Detection and enumeration of Escherichia coli  — Part 2: Miniaturised method (Most Probable Number) by inoculation in liquid medium

CSS99050-3  

EColi

Soils, sludges and treated bio-wastes — Detection and enumeration of Escherichia coli  — Part 3: Macromethod (Most Probable Number) in liquid medium

CSS99051-1

Salmonella

Soils, sludges and treated bio-wastes — Detection and enumeration of Salmonella spp.  – Part 1 : Membrane filtration method for quantitative resuscitation of sub-lethally stressed bacteria (to confirm efficacy of log reduction treatment procedures)

CSS99051-2

Salmonella

Soils, sludges and treated bio-wastes — Detection and enumeration of Salmonella spp.  – Part 2 : Liquid enrichment method in Selenite
Cystine medium followed by enrichment in Rappaport – Vassiliadis medium for semi quantitative Most Probable Number (MPN) determination

CSS99051-3

Salmonella

Soils, sludges and treated bio-wastes — Detection and enumeration of Salmonella spp.  — Part 3: Presence/absence method by liquid enrichment in peptone-novobiocin medium followed by Rappaport Vassiliadis medium

Part 1

Clostridium

 Soils, sludges and treated bio-wastes — Isolation and enumeration of Clostridium perfringens – Part 1: Membrane filtration method onto selective agar.

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Part 2

Clostridium

Soils, sludges and treated bio-wastes — Detection of Clostridium perfringens – Part 2: Macromethod (Most Probable Number) by inoculation into selective liquid medium

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Part 1

Enterococci

Soils, sludges and treated bio-wastes — Isolation and enumeration of intestinal enterococci  – Part 1: Membrane filtration method onto selective agar 

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Part 2

Enterococci

Soils, sludges and treated bio-wastes — Detection and enumeration of intestinal enterococci  – Part 2: Miniaturised method (Most Probable Number) by inoculation in liquid medium

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Part 1

Helminth Ova

Soils, sludges and treated biowastes – Detection and enumeration of viable helminth ova  - Flotation method on calcium nitrate solution, specific gravity 1.35

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Part 2

Helminth Ova

Soils, sludges and treated biowastes – Detection and enumeration of viable helminth ova - Flotation method on Natrium nitrate NaNO3 solution, specific gravity 1.35

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Part 3

Helminth Ova

Soils, sludges and treated biowastes – Detection and enumeration of viable helminth ova  - Flotation method on Zinc sulphate ZnSO4 solution, specific gravity 1.35

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Bacteriophages

Soils, sludges and treated bio-wastes — Extraction of bacteriophages.

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Plantpathogens

Soil, sludge and treated biowaste – Literature review on
detection and eradication of plant pathogens in sludge, soils and treated biowaste

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D3.4

Process validation

Methods for the validation of biotechnological, thermal and chemical processes for the treatment of animal by-products, sewage sludge and biowastes in order to determine the hygienic safety of the resulting fertilizers or comparable products by exposition of test organisms or test viruses - Part 1 : Validation with vegetative bacteria

D3.4

Process validation

Methods for the validation of biotechnological, thermal and chemical processes for the treatment of animal by-products, sewage sludge and biowastes in order to determine the hygienic safety of the resulting fertilizers or comparable products by exposition of test organisms or test viruses - Part 2 : Validation with test viruses

D3.4

Process validation

Methods for the validation of biotechnological, thermal and chemical processes for the treatment of animal by-products, sewage sludge and biowastes in order to determine the hygienic safety of the resulting fertilizers or comparable products by exposition of test organisms or test viruses - Part 3 : Validation with parasites eggs

D3.4

Process validation

Methods for the validation of biotechnological, thermal and chemical processes for the treatment of animal by-products, sewage sludge and biowastes in order to determine the hygienic safety of the resulting fertilizers or comparable products by determination of the count of selected endogenous organisms in the substrate before and after processing and calculation of the reduction rate (Input-Output-Analysis)